polyclonal anti orp8 antibody Search Results


orp8 gtx121273 antibody  (GeneTex)
90
GeneTex orp8 gtx121273 antibody
Orp8 Gtx121273 Antibody, supplied by GeneTex, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/polyclonal+anti+orp8+antibody/pm36130504-430-17-18?v=GeneTex
Average 90 stars, based on 1 article reviews
orp8 gtx121273 antibody - by Bioz Stars, 2026-08
90/100 stars
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mouse anti orp8  (Santa Cruz Biotechnology)
92
Santa Cruz Biotechnology mouse anti orp8
Mouse Anti Orp8, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/polyclonal+anti+orp8+antibody/bio_rxiv__2021__11__11__468233-300-12-14?v=Santa+Cruz+Biotechnology
Average 92 stars, based on 1 article reviews
mouse anti orp8 - by Bioz Stars, 2026-08
92/100 stars
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polyclonal anti orp8 antibody  (Boster Bio)
94
Boster Bio polyclonal anti orp8 antibody
Knockdown of Atg8 and <t>Orp8</t> blocked lipophagy. A , interference efficiency was detected by Western blotting. B , the colocalization of lysosomes and LDs in fat body cells after dsAtg8 injection. Lysosomes were stained with Lyso-Tracker ( red ), and LDs were stained with BODIPY ( green ). Yellow indicates examples of Lyso-Tracker-positive structures containing LDs. The ruler represents 50 μm. Bi , statistical analysis of autolysosome numbers in ( B ). C , the levels of triglycerides in the fat body after Atg8 knockdown. D , the location of ORP8 in fat body cells. Red fluorescence represents ORP8; green fluorescence represents LDs. The scale bar is 50 μm. E , the specificity of the ORP8 antibody in the fat body was analyzed by Western blotting. F , the expression of ORP8 was analyzed by Western blotting. G and Gi , the colocalization of LDs and lysosomes was detected after Orp8 knockdown. The ruler represents 50 μm. H , the levels of triglycerides in the fat body after Orp8 knockdown. I , phenotypes after dsOrp8 injection. Bars represent 1 cm. dsGfp was used as a control. J , statistical analysis of the phenotypes in ( I ). The pupation time was sixth instar 0 h larvae to pupae. K , the average weight of pupae and pupal phenotypes. L , percentage of different phenotypes from pupae to adults. The bars indicate the mean ± SD. p values and asterisks indicate differences by two-tailed Student's t test (∗ p < 0.05, ∗∗ p < 0.01). The different lowercase letters indicate significant differences in ANOVA ( p < 0.05). LD, lipid droplet; ORP8, oxysterol-binding protein-related protein 8.
Polyclonal Anti Orp8 Antibody, supplied by Boster Bio, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/polyclonal+anti+orp8+antibody/pmc11835591-518-0-8?v=Boster+Bio
Average 94 stars, based on 1 article reviews
polyclonal anti orp8 antibody - by Bioz Stars, 2026-08
94/100 stars
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goatarabbit irdye 800cw  (LI-COR)
99
LI-COR goatarabbit irdye 800cw
Knockdown of Atg8 and <t>Orp8</t> blocked lipophagy. A , interference efficiency was detected by Western blotting. B , the colocalization of lysosomes and LDs in fat body cells after dsAtg8 injection. Lysosomes were stained with Lyso-Tracker ( red ), and LDs were stained with BODIPY ( green ). Yellow indicates examples of Lyso-Tracker-positive structures containing LDs. The ruler represents 50 μm. Bi , statistical analysis of autolysosome numbers in ( B ). C , the levels of triglycerides in the fat body after Atg8 knockdown. D , the location of ORP8 in fat body cells. Red fluorescence represents ORP8; green fluorescence represents LDs. The scale bar is 50 μm. E , the specificity of the ORP8 antibody in the fat body was analyzed by Western blotting. F , the expression of ORP8 was analyzed by Western blotting. G and Gi , the colocalization of LDs and lysosomes was detected after Orp8 knockdown. The ruler represents 50 μm. H , the levels of triglycerides in the fat body after Orp8 knockdown. I , phenotypes after dsOrp8 injection. Bars represent 1 cm. dsGfp was used as a control. J , statistical analysis of the phenotypes in ( I ). The pupation time was sixth instar 0 h larvae to pupae. K , the average weight of pupae and pupal phenotypes. L , percentage of different phenotypes from pupae to adults. The bars indicate the mean ± SD. p values and asterisks indicate differences by two-tailed Student's t test (∗ p < 0.05, ∗∗ p < 0.01). The different lowercase letters indicate significant differences in ANOVA ( p < 0.05). LD, lipid droplet; ORP8, oxysterol-binding protein-related protein 8.
Goatarabbit Irdye 800cw, supplied by LI-COR, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/polyclonal+anti+orp8+antibody/custom%40926-32211%4029472386?v=LI-COR
Average 99 stars, based on 1 article reviews
goatarabbit irdye 800cw - by Bioz Stars, 2026-08
99/100 stars
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protein 8 orp 8  (Abcam)
99
Abcam protein 8 orp 8
Knockdown of Atg8 and <t>Orp8</t> blocked lipophagy. A , interference efficiency was detected by Western blotting. B , the colocalization of lysosomes and LDs in fat body cells after dsAtg8 injection. Lysosomes were stained with Lyso-Tracker ( red ), and LDs were stained with BODIPY ( green ). Yellow indicates examples of Lyso-Tracker-positive structures containing LDs. The ruler represents 50 μm. Bi , statistical analysis of autolysosome numbers in ( B ). C , the levels of triglycerides in the fat body after Atg8 knockdown. D , the location of ORP8 in fat body cells. Red fluorescence represents ORP8; green fluorescence represents LDs. The scale bar is 50 μm. E , the specificity of the ORP8 antibody in the fat body was analyzed by Western blotting. F , the expression of ORP8 was analyzed by Western blotting. G and Gi , the colocalization of LDs and lysosomes was detected after Orp8 knockdown. The ruler represents 50 μm. H , the levels of triglycerides in the fat body after Orp8 knockdown. I , phenotypes after dsOrp8 injection. Bars represent 1 cm. dsGfp was used as a control. J , statistical analysis of the phenotypes in ( I ). The pupation time was sixth instar 0 h larvae to pupae. K , the average weight of pupae and pupal phenotypes. L , percentage of different phenotypes from pupae to adults. The bars indicate the mean ± SD. p values and asterisks indicate differences by two-tailed Student's t test (∗ p < 0.05, ∗∗ p < 0.01). The different lowercase letters indicate significant differences in ANOVA ( p < 0.05). LD, lipid droplet; ORP8, oxysterol-binding protein-related protein 8.
Protein 8 Orp 8, supplied by Abcam, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/polyclonal+anti+orp8+antibody/pm24333576-63-50-53?v=Abcam
Average 99 stars, based on 1 article reviews
protein 8 orp 8 - by Bioz Stars, 2026-08
99/100 stars
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sr-bi antibody - bsa free  (Bio-Techne corporation)
94
Bio-Techne corporation sr-bi antibody - bsa free
Knockdown of Atg8 and <t>Orp8</t> blocked lipophagy. A , interference efficiency was detected by Western blotting. B , the colocalization of lysosomes and LDs in fat body cells after dsAtg8 injection. Lysosomes were stained with Lyso-Tracker ( red ), and LDs were stained with BODIPY ( green ). Yellow indicates examples of Lyso-Tracker-positive structures containing LDs. The ruler represents 50 μm. Bi , statistical analysis of autolysosome numbers in ( B ). C , the levels of triglycerides in the fat body after Atg8 knockdown. D , the location of ORP8 in fat body cells. Red fluorescence represents ORP8; green fluorescence represents LDs. The scale bar is 50 μm. E , the specificity of the ORP8 antibody in the fat body was analyzed by Western blotting. F , the expression of ORP8 was analyzed by Western blotting. G and Gi , the colocalization of LDs and lysosomes was detected after Orp8 knockdown. The ruler represents 50 μm. H , the levels of triglycerides in the fat body after Orp8 knockdown. I , phenotypes after dsOrp8 injection. Bars represent 1 cm. dsGfp was used as a control. J , statistical analysis of the phenotypes in ( I ). The pupation time was sixth instar 0 h larvae to pupae. K , the average weight of pupae and pupal phenotypes. L , percentage of different phenotypes from pupae to adults. The bars indicate the mean ± SD. p values and asterisks indicate differences by two-tailed Student's t test (∗ p < 0.05, ∗∗ p < 0.01). The different lowercase letters indicate significant differences in ANOVA ( p < 0.05). LD, lipid droplet; ORP8, oxysterol-binding protein-related protein 8.
Sr Bi Antibody Bsa Free, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/polyclonal+anti+orp8+antibody/custom%40nb400-101%4023554939?v=Bio-Techne+corporation
Average 94 stars, based on 1 article reviews
sr-bi antibody - bsa free - by Bioz Stars, 2026-08
94/100 stars
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abca1 antibody - bsa free  (Bio-Techne corporation)
95
Bio-Techne corporation abca1 antibody - bsa free
Knockdown of Atg8 and <t>Orp8</t> blocked lipophagy. A , interference efficiency was detected by Western blotting. B , the colocalization of lysosomes and LDs in fat body cells after dsAtg8 injection. Lysosomes were stained with Lyso-Tracker ( red ), and LDs were stained with BODIPY ( green ). Yellow indicates examples of Lyso-Tracker-positive structures containing LDs. The ruler represents 50 μm. Bi , statistical analysis of autolysosome numbers in ( B ). C , the levels of triglycerides in the fat body after Atg8 knockdown. D , the location of ORP8 in fat body cells. Red fluorescence represents ORP8; green fluorescence represents LDs. The scale bar is 50 μm. E , the specificity of the ORP8 antibody in the fat body was analyzed by Western blotting. F , the expression of ORP8 was analyzed by Western blotting. G and Gi , the colocalization of LDs and lysosomes was detected after Orp8 knockdown. The ruler represents 50 μm. H , the levels of triglycerides in the fat body after Orp8 knockdown. I , phenotypes after dsOrp8 injection. Bars represent 1 cm. dsGfp was used as a control. J , statistical analysis of the phenotypes in ( I ). The pupation time was sixth instar 0 h larvae to pupae. K , the average weight of pupae and pupal phenotypes. L , percentage of different phenotypes from pupae to adults. The bars indicate the mean ± SD. p values and asterisks indicate differences by two-tailed Student's t test (∗ p < 0.05, ∗∗ p < 0.01). The different lowercase letters indicate significant differences in ANOVA ( p < 0.05). LD, lipid droplet; ORP8, oxysterol-binding protein-related protein 8.
Abca1 Antibody Bsa Free, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/polyclonal+anti+orp8+antibody/custom%40nb400-105%4023554939?v=Bio-Techne+corporation
Average 95 stars, based on 1 article reviews
abca1 antibody - bsa free - by Bioz Stars, 2026-08
95/100 stars
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mouse anti-gapdh gtx627408  (GeneTex)
90
GeneTex mouse anti-gapdh gtx627408
Knockdown of Atg8 and <t>Orp8</t> blocked lipophagy. A , interference efficiency was detected by Western blotting. B , the colocalization of lysosomes and LDs in fat body cells after dsAtg8 injection. Lysosomes were stained with Lyso-Tracker ( red ), and LDs were stained with BODIPY ( green ). Yellow indicates examples of Lyso-Tracker-positive structures containing LDs. The ruler represents 50 μm. Bi , statistical analysis of autolysosome numbers in ( B ). C , the levels of triglycerides in the fat body after Atg8 knockdown. D , the location of ORP8 in fat body cells. Red fluorescence represents ORP8; green fluorescence represents LDs. The scale bar is 50 μm. E , the specificity of the ORP8 antibody in the fat body was analyzed by Western blotting. F , the expression of ORP8 was analyzed by Western blotting. G and Gi , the colocalization of LDs and lysosomes was detected after Orp8 knockdown. The ruler represents 50 μm. H , the levels of triglycerides in the fat body after Orp8 knockdown. I , phenotypes after dsOrp8 injection. Bars represent 1 cm. dsGfp was used as a control. J , statistical analysis of the phenotypes in ( I ). The pupation time was sixth instar 0 h larvae to pupae. K , the average weight of pupae and pupal phenotypes. L , percentage of different phenotypes from pupae to adults. The bars indicate the mean ± SD. p values and asterisks indicate differences by two-tailed Student's t test (∗ p < 0.05, ∗∗ p < 0.01). The different lowercase letters indicate significant differences in ANOVA ( p < 0.05). LD, lipid droplet; ORP8, oxysterol-binding protein-related protein 8.
Mouse Anti Gapdh Gtx627408, supplied by GeneTex, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/polyclonal+anti+orp8+antibody/bio_rxiv__2021__11__11__468233-300-27-29?v=GeneTex
Average 90 stars, based on 1 article reviews
mouse anti-gapdh gtx627408 - by Bioz Stars, 2026-08
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anti-sec22b  (Synaptic Systems)
90
Synaptic Systems anti-sec22b
Knockdown of Atg8 and <t>Orp8</t> blocked lipophagy. A , interference efficiency was detected by Western blotting. B , the colocalization of lysosomes and LDs in fat body cells after dsAtg8 injection. Lysosomes were stained with Lyso-Tracker ( red ), and LDs were stained with BODIPY ( green ). Yellow indicates examples of Lyso-Tracker-positive structures containing LDs. The ruler represents 50 μm. Bi , statistical analysis of autolysosome numbers in ( B ). C , the levels of triglycerides in the fat body after Atg8 knockdown. D , the location of ORP8 in fat body cells. Red fluorescence represents ORP8; green fluorescence represents LDs. The scale bar is 50 μm. E , the specificity of the ORP8 antibody in the fat body was analyzed by Western blotting. F , the expression of ORP8 was analyzed by Western blotting. G and Gi , the colocalization of LDs and lysosomes was detected after Orp8 knockdown. The ruler represents 50 μm. H , the levels of triglycerides in the fat body after Orp8 knockdown. I , phenotypes after dsOrp8 injection. Bars represent 1 cm. dsGfp was used as a control. J , statistical analysis of the phenotypes in ( I ). The pupation time was sixth instar 0 h larvae to pupae. K , the average weight of pupae and pupal phenotypes. L , percentage of different phenotypes from pupae to adults. The bars indicate the mean ± SD. p values and asterisks indicate differences by two-tailed Student's t test (∗ p < 0.05, ∗∗ p < 0.01). The different lowercase letters indicate significant differences in ANOVA ( p < 0.05). LD, lipid droplet; ORP8, oxysterol-binding protein-related protein 8.
Anti Sec22b, supplied by Synaptic Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/polyclonal+anti+orp8+antibody/pmc10550925__42003_2023_5382_MOESM2_ESM-23-28-29?v=Synaptic+Systems
Average 90 stars, based on 1 article reviews
anti-sec22b - by Bioz Stars, 2026-08
90/100 stars
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rabbit anti orp5  (Atlas Antibodies)
92
Atlas Antibodies rabbit anti orp5
Knockdown of Atg8 and <t>Orp8</t> blocked lipophagy. A , interference efficiency was detected by Western blotting. B , the colocalization of lysosomes and LDs in fat body cells after dsAtg8 injection. Lysosomes were stained with Lyso-Tracker ( red ), and LDs were stained with BODIPY ( green ). Yellow indicates examples of Lyso-Tracker-positive structures containing LDs. The ruler represents 50 μm. Bi , statistical analysis of autolysosome numbers in ( B ). C , the levels of triglycerides in the fat body after Atg8 knockdown. D , the location of ORP8 in fat body cells. Red fluorescence represents ORP8; green fluorescence represents LDs. The scale bar is 50 μm. E , the specificity of the ORP8 antibody in the fat body was analyzed by Western blotting. F , the expression of ORP8 was analyzed by Western blotting. G and Gi , the colocalization of LDs and lysosomes was detected after Orp8 knockdown. The ruler represents 50 μm. H , the levels of triglycerides in the fat body after Orp8 knockdown. I , phenotypes after dsOrp8 injection. Bars represent 1 cm. dsGfp was used as a control. J , statistical analysis of the phenotypes in ( I ). The pupation time was sixth instar 0 h larvae to pupae. K , the average weight of pupae and pupal phenotypes. L , percentage of different phenotypes from pupae to adults. The bars indicate the mean ± SD. p values and asterisks indicate differences by two-tailed Student's t test (∗ p < 0.05, ∗∗ p < 0.01). The different lowercase letters indicate significant differences in ANOVA ( p < 0.05). LD, lipid droplet; ORP8, oxysterol-binding protein-related protein 8.
Rabbit Anti Orp5, supplied by Atlas Antibodies, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/polyclonal+anti+orp8+antibody/pmc11021183-232-39-41?v=Atlas+Antibodies
Average 92 stars, based on 1 article reviews
rabbit anti orp5 - by Bioz Stars, 2026-08
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pdi gtx30716 antibody  (GeneTex)
90
GeneTex pdi gtx30716 antibody
Knockdown of Atg8 and <t>Orp8</t> blocked lipophagy. A , interference efficiency was detected by Western blotting. B , the colocalization of lysosomes and LDs in fat body cells after dsAtg8 injection. Lysosomes were stained with Lyso-Tracker ( red ), and LDs were stained with BODIPY ( green ). Yellow indicates examples of Lyso-Tracker-positive structures containing LDs. The ruler represents 50 μm. Bi , statistical analysis of autolysosome numbers in ( B ). C , the levels of triglycerides in the fat body after Atg8 knockdown. D , the location of ORP8 in fat body cells. Red fluorescence represents ORP8; green fluorescence represents LDs. The scale bar is 50 μm. E , the specificity of the ORP8 antibody in the fat body was analyzed by Western blotting. F , the expression of ORP8 was analyzed by Western blotting. G and Gi , the colocalization of LDs and lysosomes was detected after Orp8 knockdown. The ruler represents 50 μm. H , the levels of triglycerides in the fat body after Orp8 knockdown. I , phenotypes after dsOrp8 injection. Bars represent 1 cm. dsGfp was used as a control. J , statistical analysis of the phenotypes in ( I ). The pupation time was sixth instar 0 h larvae to pupae. K , the average weight of pupae and pupal phenotypes. L , percentage of different phenotypes from pupae to adults. The bars indicate the mean ± SD. p values and asterisks indicate differences by two-tailed Student's t test (∗ p < 0.05, ∗∗ p < 0.01). The different lowercase letters indicate significant differences in ANOVA ( p < 0.05). LD, lipid droplet; ORP8, oxysterol-binding protein-related protein 8.
Pdi Gtx30716 Antibody, supplied by GeneTex, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/polyclonal+anti+orp8+antibody/pm36130504-371-59-60?v=GeneTex
Average 90 stars, based on 1 article reviews
pdi gtx30716 antibody - by Bioz Stars, 2026-08
90/100 stars
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rabbit anti mfn2  (Proteintech)
96
Proteintech rabbit anti mfn2
Knockdown of Atg8 and <t>Orp8</t> blocked lipophagy. A , interference efficiency was detected by Western blotting. B , the colocalization of lysosomes and LDs in fat body cells after dsAtg8 injection. Lysosomes were stained with Lyso-Tracker ( red ), and LDs were stained with BODIPY ( green ). Yellow indicates examples of Lyso-Tracker-positive structures containing LDs. The ruler represents 50 μm. Bi , statistical analysis of autolysosome numbers in ( B ). C , the levels of triglycerides in the fat body after Atg8 knockdown. D , the location of ORP8 in fat body cells. Red fluorescence represents ORP8; green fluorescence represents LDs. The scale bar is 50 μm. E , the specificity of the ORP8 antibody in the fat body was analyzed by Western blotting. F , the expression of ORP8 was analyzed by Western blotting. G and Gi , the colocalization of LDs and lysosomes was detected after Orp8 knockdown. The ruler represents 50 μm. H , the levels of triglycerides in the fat body after Orp8 knockdown. I , phenotypes after dsOrp8 injection. Bars represent 1 cm. dsGfp was used as a control. J , statistical analysis of the phenotypes in ( I ). The pupation time was sixth instar 0 h larvae to pupae. K , the average weight of pupae and pupal phenotypes. L , percentage of different phenotypes from pupae to adults. The bars indicate the mean ± SD. p values and asterisks indicate differences by two-tailed Student's t test (∗ p < 0.05, ∗∗ p < 0.01). The different lowercase letters indicate significant differences in ANOVA ( p < 0.05). LD, lipid droplet; ORP8, oxysterol-binding protein-related protein 8.
Rabbit Anti Mfn2, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/polyclonal+anti+orp8+antibody/bio_rxiv__2025__11__06__685814-393-57-59?v=Proteintech
Average 96 stars, based on 1 article reviews
rabbit anti mfn2 - by Bioz Stars, 2026-08
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Image Search Results


Knockdown of Atg8 and Orp8 blocked lipophagy. A , interference efficiency was detected by Western blotting. B , the colocalization of lysosomes and LDs in fat body cells after dsAtg8 injection. Lysosomes were stained with Lyso-Tracker ( red ), and LDs were stained with BODIPY ( green ). Yellow indicates examples of Lyso-Tracker-positive structures containing LDs. The ruler represents 50 μm. Bi , statistical analysis of autolysosome numbers in ( B ). C , the levels of triglycerides in the fat body after Atg8 knockdown. D , the location of ORP8 in fat body cells. Red fluorescence represents ORP8; green fluorescence represents LDs. The scale bar is 50 μm. E , the specificity of the ORP8 antibody in the fat body was analyzed by Western blotting. F , the expression of ORP8 was analyzed by Western blotting. G and Gi , the colocalization of LDs and lysosomes was detected after Orp8 knockdown. The ruler represents 50 μm. H , the levels of triglycerides in the fat body after Orp8 knockdown. I , phenotypes after dsOrp8 injection. Bars represent 1 cm. dsGfp was used as a control. J , statistical analysis of the phenotypes in ( I ). The pupation time was sixth instar 0 h larvae to pupae. K , the average weight of pupae and pupal phenotypes. L , percentage of different phenotypes from pupae to adults. The bars indicate the mean ± SD. p values and asterisks indicate differences by two-tailed Student's t test (∗ p < 0.05, ∗∗ p < 0.01). The different lowercase letters indicate significant differences in ANOVA ( p < 0.05). LD, lipid droplet; ORP8, oxysterol-binding protein-related protein 8.

Journal: The Journal of Biological Chemistry

Article Title: The steroid hormone 20-hydroxyecdysone induces lipophagy via the brain-adipose tissue axis by promoting the adipokinetic hormone pathway

doi: 10.1016/j.jbc.2025.108179

Figure Lengend Snippet: Knockdown of Atg8 and Orp8 blocked lipophagy. A , interference efficiency was detected by Western blotting. B , the colocalization of lysosomes and LDs in fat body cells after dsAtg8 injection. Lysosomes were stained with Lyso-Tracker ( red ), and LDs were stained with BODIPY ( green ). Yellow indicates examples of Lyso-Tracker-positive structures containing LDs. The ruler represents 50 μm. Bi , statistical analysis of autolysosome numbers in ( B ). C , the levels of triglycerides in the fat body after Atg8 knockdown. D , the location of ORP8 in fat body cells. Red fluorescence represents ORP8; green fluorescence represents LDs. The scale bar is 50 μm. E , the specificity of the ORP8 antibody in the fat body was analyzed by Western blotting. F , the expression of ORP8 was analyzed by Western blotting. G and Gi , the colocalization of LDs and lysosomes was detected after Orp8 knockdown. The ruler represents 50 μm. H , the levels of triglycerides in the fat body after Orp8 knockdown. I , phenotypes after dsOrp8 injection. Bars represent 1 cm. dsGfp was used as a control. J , statistical analysis of the phenotypes in ( I ). The pupation time was sixth instar 0 h larvae to pupae. K , the average weight of pupae and pupal phenotypes. L , percentage of different phenotypes from pupae to adults. The bars indicate the mean ± SD. p values and asterisks indicate differences by two-tailed Student's t test (∗ p < 0.05, ∗∗ p < 0.01). The different lowercase letters indicate significant differences in ANOVA ( p < 0.05). LD, lipid droplet; ORP8, oxysterol-binding protein-related protein 8.

Article Snippet: Polyclonal anti-ORP8 antibody (anti-OSBPL8 antibody) was obtained from Boster Biological Technology.

Techniques: Knockdown, Western Blot, Injection, Staining, Fluorescence, Expressing, Control, Two Tailed Test, Binding Assay